I-Cpressure Sterilization ye-Chemical Biological Indicator
Incazelo emfushane:
Lo mkhiqizo uqukethe inkomba yebhayoloji eziqukathe ngokwayo eyakhiwe ngezinhlamvu ze-Bacillus stearothermophilus, i-culture medium (evalwe ngeshubhu lengilazi) kanye negobolondo lepulasitiki.Okuqukethwe kwebhaktheriya kwezingcezu zebhaktheriya ngu-5 × 105~ 5 × 106cfu / ucezu.D inani liyimizuzu engu-1.3 ~ 1.9.Ngaphansi kwesimo se-121 ℃ ± 0.5 ℃ esigcwele isitimu, isikhathi sokusinda singamaminithi angu-≥3.9 futhi isikhathi sokubulala imizuzu engu-≤19.
Ububanzi bohlelo lokusebenza
Isetshenziselwa ukuqapha umphumela wokuvala inzalo we-down-exhaust pressure steam ku-121 ℃, i-pre-vacuum pressure steam ku-132 ℃ kanye ne-pulsating vacuum pressure steam.
Ukusetshenziswa
1.Faka lo mkhiqizo ephaketheni lokuhlola elijwayelekile;
2.Ngokwemithetho kazwelonke, beka iphakethe lokuhlola ezindaweni ezihlukene kumshini wokukhipha umusi wokucindezela;
3.Ngemva kokuvala inzalo, susa inkomba yezinto eziphilayo;
4. Cindezela ishubhu yengilazi ngaphakathi bese ufaka inkomba ku-incubator engu-56 ℃ -58 ℃ kanye neshubhu lokulawula;
5.Ukunqunywa kwemiphumela ngemva kokutshala amahora angu-48: umbala we-medium ushintsha kusuka kokunsomi ukuya kophuzi, okubonisa ukuthi inqubo yokuvala inzalo ayiphelele.Uma umbala we-culture medium uhlala ungashintshiwe, kungahlulelwa ukuthi ukuvala inzalo kuphelile.
Izixwayiso
1.Ngemva kokuvala inzalo, susa inkomba yebhayoloji bese uyipholisa okungenani imizuzu eyi-15 ngaphambi kokukhama ishubhu lengilazi elingaphakathi.Uma kungenjalo, izingcezu zeshubhu yengilazi zingadala ukulimala.
I-2.I-tube yokulawula kuphela enhle, umphumela wokuhlolwa kwezinto eziphilayo ubhekwa njengokuvumelekile.
3.Ngaphambi kokusebenzisa, sicela uqinisekise ubuqotho bomkhiqizo.
4.Sicela ugcine endaweni emnyama emazingeni okushisa angama-2-25 ° C kanye nomswakama ohlobene no-20% -80%.
5.Izinkomba zebhayoloji kufanele zigcinwe kude nezibulala-magciwane nezibulala magciwane ngamakhemikhali.
6.Sicela usebenzise phakathi nesikhathi sokuqinisekisa.
7.Isikhathi esivumelekile : Izinyanga ezingama-24
